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MONKEYPOX MICROTEACHING Monkeypox is diagnosed by PCR test on a viral swab taken from one or more vesicles or ulcers, or from a dry scraping of the scab. Swabs should be sent in viral transport media. Scab scrapings should only be taken if there are no other lesions and should be sent in a standard universal container. Testing of scrapings may take longer than a lesion swab. A. QUESTION Why we need to ensure that we are wearing the minimum appropriate PPE while diagnosing monkeypox procedures? B. EXPLAIN THE FOLLOWING PROCEDURES! If other wounds are present, ensure that the sample is definitely taken from an ulcer, vesicle, or crusted vesicle. Rub the swab over the lesion and place the swab in the viral culture tube or viral transport medium. Label the tube with the patient’s name and date of birth, the date and site of the sample. Please note that unlabeled tubes cannot be processed. If all the lesions are crusted, scrape scab material into a dry plain universal container and label as above. If the patient has fever or widespread rash, sore throat or other systemic symptoms, you should also take an EDTA blood sample and a throat swab. Urine samples may also be sent in a universal container and will be tested if deemed necessary for clinical management. For high risk contacts of a confirmed case who have developed systemic symptoms but do not have a rash or lesions for sampling, you should take a throat swab in viral transport media. Note that even if the throat swab is negative, the individual must continue with monitoring and isolation as instructed, and should be reassessed and sampled if further symptoms develop Samples for investigation of other infections, including sexually transmitted infections, should be packaged separately, with separate request forms. Samples should be sent to your normal local laboratory for forwarding to the Rare and Imported Pathogens Laboratory (RIPL) (for monkeypox testing) and for local processing (all other tests) Samples from suspected cases of monkeypox should be sent by Category B transport.
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